Thermo Fisher Scientific MCP-1 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine JE/MCP-1 (CCL2). Anti-Murine JE/MCP-1 (CCL2)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Murine JE/MCP-1 (CCL2) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine JE/MCP-1 (CCL2) (500-P113) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine JE/MCP-1 (CCL2).

Western Blot:
To detect mJE(MCP-1) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant mJE(MCP-1) is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P113BT-1MG will be provided as 2 × 500 µg.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are secreted proteins involved in immunoregulatory and inflammatory processes. The encoded protein is structurally related to the CXC subfamily of cytokines, characterized by two cysteines separated by one amino acid. It displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.