Thermo Fisher Scientific CXCL1 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• 권장 농도: 0.1–0.2 µg/mL

ELISA

• 권장 농도: 0.25–1.0 µg/mL
• Sandwich ELISA에서 최소 0.2–0.4 ng/well 검출 가능

Miscellaneous (PubMed)

• 참고 문헌 있음

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine KC (CXCL1). Anti-Murine KC (CXCL1)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Murine KC (CXCL1) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine KC (CXCL1) (500-P115) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Murine KC (CXCL1).

Western Blot:
To detect mKC by Western blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used in conjunction with compatible secondary reagents, the detection limit for Recombinant mKC is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P115BT-1MG will be provided as 2 × 500 µg.

Target Information

CXCL1, CXCL2, and CXCL3 (also known as GRO alpha, GRO beta, and GRO gamma) are members of the CXC chemokine subfamily.
Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators.
In vitro, the processed forms GRO-alpha(4–73), GRO-alpha(5–73), and GRO-alpha(6–73) show a 30-fold higher chemotactic activity.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.