Thermo Fisher Scientific CCL21 Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Immunostaining (IS)

-

View 6 publications 6 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine Exodus-2 (CCL21) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CCL21,uniProtId:P86793-1,ncbiNodeId:10090,antigenRange:24-133,antigenLength:133,antigenImageFileName:500-P114-100UG_CCL21_P86793-1_Rabbit.svg,antigenImageFileNamePDP:500-P114-100UG_CCL21_P86793-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929275

Product Specific Information

AA Sequence of recombinant protein: SDGGGQDCCL KYSQKKIPYS IVRGYRKQEP SLGCPIPAIL FLPRKHSKPE LCANPEEGWV QNLMRRLDQP PAPGKQSPGC RKNRGTSKSG KKGKGSKGCK RTEQTQPSRG.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine Exodus-2 (CCL21). Anti-Murine Exodus-2 (CCL21)-specific antibody was purified by affinity chromatography employing an immobilized Murine Exodus-2 (CCL21) matrix.

Sandwich ELISA: To detect Murine Exodus-2 (CCL21) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine Exodus-2 (CCL21) (500-P114Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine Exodus-2 (CCL21).

Western Blot: To detect Murine Exodus-2 (CCL21) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine Exodus-2 (CCL21) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.