Thermo Fisher Scientific iNOS Monoclonal Antibody (CXNFT), PE-Cyanine5.5, eBioscience

Thermo Fisher Scientific iNOS Monoclonal Antibody (CXNFT), PE-Cyanine5.5, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.125 µg/test
  View 31 publications

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Brilliant UV 395, 563, 615, 737
• Brilliant Violet 421, 480, 605, 786
• eFluor 450
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). Nitric oxide synthase enzymes catalyze the formation of nitric oxide from L-arginine through an NADPH- and oxygen-dependent mechanism.
There are three isoforms of NOS encoded by separate genes: NOS1 (nNOS), NOS2 (iNOS), and NOS3 (eNOS). NOS2 is induced by bacterial endotoxins and inflammatory cytokines (IFN-γ, TNF-α). It is expressed in myeloid-derived suppressor cells and M1 macrophages but not M2 macrophages.

• Reported Applications: Intracellular staining followed by flow cytometric analysis
• Tested Applications: Intracellular staining of stimulated thioglycolate-elicited peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00)
• Recommended ≤ 0.125 µg per test (100 µL final volume; 10⁵–10⁸ cells/test)
• Carefully titrate for optimal assay performance

Light Sensitivity: Tandem dye is sensitive to photo-induced oxidation. Protect vial and samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in dark using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57).
Excitation: 488–561 nm; Emission: 695 nm; Laser: Blue, Green, Yellow-Green.

Target Information

iNOS (Inducible Nitric Oxide Synthase) produces nitric oxide (NO), a gaseous free radical involved in vasorelaxation, neurotransmission, and cytotoxicity.
NO production is mediated by NOS family enzymes. iNOS is inducible by lipopolysaccharides and cytokines and expressed in liver, macrophages, hepatocytes, synoviocytes, and smooth muscle cells.
After cytokine induction, iNOS shows delayed activation followed by sustained NO production.
Three iNOS pseudogenes are located on chromosome 17 (Smith-Magenis syndrome region).
Diseases associated with iNOS dysfunction include achalasia and impotence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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