Thermo Fisher Scientific Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), PE-Cyanine5.5, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin, Brilliant UV Series (395–805)
• Brilliant Violet Series (421–786)
• eFluor 450, 506, 660
• FITC, Functional Grade
• NovaFluor Red 700, NovaFluor Yellow 610
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright Series (436–780)
• Request custom conjugation

Product Specific Information

The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21–25 kDa GPI-linked protein (Gr-1 antigen) expressed in the myeloid lineage during bone marrow development.

• Monocytes transiently express Gr-1 during development.
• Granulocytes and peripheral neutrophils show strong Gr-1 expression, serving as reliable markers for these cell populations.

eBioscience testing indicates that this antibody also stains cells expressing high Ly6C levels. For Ly-6G-specific detection, use 1A8-Ly6G (Product #9668).

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse bone marrow and splenocyte suspensions

• Recommended ≤0.125 µg per test (per 100 µL sample)
• Cell number: 10⁵–10⁸ cells/test (empirically determined)
• Optimize antibody titration for best performance

Light Sensitivity: Protect from light; tandem dye is photo-sensitive.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)

Fluorescence Characteristics:

• Excitation: 488–561 nm
• Emission: 695 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Target Information

Granulocytes are white blood cells containing cytoplasmic granules and are also known as polymorphonuclear leukocytes (PMN/PML).
Neutrophil granulocytes are the most abundant type and play a key role in immune defense.
Granulocytes are released from bone marrow under the regulation of complement proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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