Thermo Fisher Scientific Epo Polyclonal Antibody, PeproTech
Human EPO를 검출하기 위한 Rabbit Polyclonal Antibody로, Western Blot과 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 고순도 재조합 인간 EPO로 면역화된 혈청에서 생산되었습니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 500-P318-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Epo Polyclonal Antibody, PeproTech
Applications
Western Blot (WB)
- Tested Dilution: 0.1–0.2 µg/mL
ELISA
- Tested Dilution: 0.5–2.0 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHO cells-derived Recombinant Human EPO |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2930365 |
Product Specific Information
AA Sequence of recombinant protein:
APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human EPO. Anti-Human EPO-specific antibody was purified by affinity chromatography employing an immobilized Human EPO matrix.
Sandwich ELISA:
To detect Human EPO by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human EPO (500-P318Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human EPO.
Western Blot:
To detect Human EPO by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human EPO is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO induces the final maturation of partially differentiated erythroid cells and promotes production of red cell-specific proteins such as hemoglobin.
The EPO receptor (EPOR) is highly expressed in precursor cells and decreases with maturation. Hepatocytes are the main source of EPO in the fetus, while renal interstitial cells become the major source after birth. EPO expression increases under hypoxic conditions and is also detected in lung and brain tissue.
Recent studies suggest EPO may contribute to neuronal tissue protection and repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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