Thermo Fisher Scientific Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor 488

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 3 publications 3 publications

Immunohistochemistry - Free Floating (IHC (Free))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-5 µg/mL

View 10 publications 10 publications

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Bovine, Dog, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

OC-3F10

Immunogen

GST-fusion protein consisting of the C-terminal region (~150 aa) of human occludin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Occludin,uniProtId:Q16625-1,ncbiNodeId:9606,antigenRange:150,antigenLength:522,antigenImageFileName:331588_Occludin_Q16625-1_House_mouse.svg,antigenImageFileNamePDP:331588_Occludin_Q16625-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• Unconjugated
• Alexa Fluor 594
• FITC
• HRP
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 4.0mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532185

Product Specific Information

Reactivity of this antibody with the occludin protein has been confirmed by immunofluorescence and western blotting (unconjugated). This antibody reacts specifically with mammalian occludin. Tissues/lysates that were tested with this antibody include T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma), MTE7B (Mouse), and rat liver.

Target Information

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.