Thermo Fisher Scientific Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor 488
Alexa Fluor 488 형광이 결합된 Occludin 단클론 항체로, 포유류 Occludin 단백질을 특이적으로 인식합니다. Western blot, IHC, ICC 등 다양한 응용에 사용 가능하며, 고순도 Protein A 정제 및 안정적 액상 형태로 제공됩니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor 488
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 2 publications |
| Immunohistochemistry (IHC) | - | View 5 publications |
| Immunohistochemistry (PFA fixed) | - | View 1 publication |
| Immunohistochemistry (Frozen) | - | View 3 publications |
| Immunohistochemistry - Free Floating | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1–5 µg/mL | View 10 publications |
| ELISA | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Published Species | Bovine, Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OC-3F10 |
| Immunogen | GST-fusion protein consisting of the C-terminal region (~150 aa) of human occludin |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 4.0 mg/mL BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2532185 |
Additional Formats
Product Specific Information
Reactivity of this antibody with occludin protein has been confirmed by immunofluorescence and western blotting (unconjugated). This antibody reacts specifically with mammalian occludin. Tested tissues/lysates include:
- T84 cell line (human intestinal epithelium)
- MDCK cells (canine kidney)
- Caco-2 cells (human colon adenocarcinoma)
- MTE7B (mouse)
- Rat liver
Target Information
Occludin is a 65 kDa protein that can exist in phosphorylated forms up to ~82 kDa. It regulates localization and function of tight junctions. Polyunsaturated fatty acids up-regulate occludin expression, enhancing transendothelial resistance and reducing permeability. Occludin expression varies by tissue—highly expressed in brain, lower and discontinuous in non-neural tissues. Under-expression of occludin contributes to increased permeability in tumor endothelial junctions, associated with brain tumor edema.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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