Thermo Fisher Scientific CD369 (Clec7a, Dectin-1) Monoclonal Antibody (bg1fpj), PerCP-eFluor 710, eBioscience
마우스 CD369(Dectin-1, CLEC7A)을 인식하는 단클론 항체로, PerCP-eFluor 710 형광체가 결합된 제품입니다. Flow Cytometry 분석에 적합하며, 0.125 µg/test 농도로 사용 가능합니다. 면역세포의 Th1/Th17 분화 및 사이토카인 생성 연구에 유용합니다.
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Thermo Fisher Scientific CD369 (Clec7a, Dectin-1) Monoclonal Antibody (bg1fpj), PerCP-eFluor 710
Applications and Tested Dilution
- Application: Flow Cytometry
- Tested Dilution: 0.125 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | bg1fpj |
| Immunogen | Baculovirus-expressed Fc-containing protein |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573779 |
Additional Formats
- APC
- PE
- PE-Cyanine7
- Custom conjugation available
Product Specific Information
The monoclonal antibody bg1fpj recognizes mouse CD369 (Dectin-1, CLEC7A), a type II transmembrane protein in the C-type lectin family.
CD369 binds β1,3-glucans found in fungal, plant, and bacterial cell walls. It mediates calcium-dependent signaling via Syk through the ITAM domain, leading to endocytosis/phagocytosis and cytokine production (IL-2, IL-6, IL-10, TNFα, IL-1β, IL-23).
CD369 signaling is linked to Th17 and Th1 differentiation. It is mainly expressed on myeloid cells (neutrophils, monocytes, macrophages, dendritic cells) and at low levels on lymphocytes and mucosal epithelium.
Applications Reported
- Reported for use in flow cytometric analysis.
Applications Tested
- Tested in flow cytometric analysis of mouse peripheral blood cells.
- Recommended usage ≤ 0.125 µg/test (100 µL final volume).
- Cell number: 10⁵–10⁸ cells/test.
- Titrate for optimal performance.
Fluorochrome Information
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm).
- Recommended filter: 710/50 bandpass (alternative: 695/40 bandpass).
- Ensure instrument compatibility.
Fixation
Samples can be stored in:
- IC Fixation Buffer (Product #00-8222-49)
- 1-step Fix/Lyse Solution (Product #00-5333-54)
Store up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Optical Properties
- Excitation: 488 nm
- Emission: 710 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CLEC7A (Dectin-1) is a C-type lectin receptor expressed mainly on myeloid cells.
It recognizes β-1,3 and β-1,6 glucans from fungi and plants, triggering innate immune responses via Syk tyrosine kinase activation and reactive oxygen species formation.
For Research Use Only. Not for use in diagnostic procedures.
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