Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), NovaFluor Blue 690, eBioscience

Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), NovaFluor Blue 690

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.2 µg)/test

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Product Specifications

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Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Brilliant UV 496, 615, 737, 805
• Brilliant Violet 421, 480, 650, 711, 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510, 555, 585, 610-30S, 660-40S, 760
• NovaFluor Red 710, 725, 755
• NovaFluor Yellow 570, 590, 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600
• Request custom conjugation

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Product Specific Information

The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells. It binds glycosylated, fucosylated, and sulfated sialylated glycoproteins such as CD34, GlyCAM-1, and MAdCAM-1, mediating lymphocyte rolling and homing to high endothelial venules (HEV).

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells; use at 5 µL (1 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

NovaFluor Compatibility:
Not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for NovaFluor dyes.

CellBlox Plus Blocking Buffer:
Required for optimal staining with NovaFluor conjugates.
Add 5 µL per sample to antibody cocktails/master mixes before combining with cells.
For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates use Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescent signatures minimizing spillover and spread.

Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

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Target Information

Human CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor with:

• Aminoterminal C-type lectin domain
• EGF-like domain
• Two short consensus repeat (SCR) sequences
• Transmembrane and cytoplasmic regions

It is constitutively expressed on all classes of leukocytes including lymphocytes (except many memory T cells), monocytes, and polymorphonuclear cells.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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