Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), NovaFluor Blue 660-40S, eBioscience

Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), NovaFluor Blue 660-40S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.2 µg)/test

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Product Specifications

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Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Brilliant UV 496 / 615 / 737 / 805
• Brilliant Violet 421 / 480 / 650 / 711 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 555 / 585 / 610-30S / 690 / 760
• NovaFluor Red 710 / 725 / 755
• NovaFluor Yellow 570 / 590 / 660
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600
• Custom conjugation available

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Product Specific Information

The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells. It binds glycosylated, fucosylated, sulfated sialylated glycoproteins such as CD34, glycam-1, and MAdCAM-1, mediating lymphocyte rolling on activated endothelium and homing to peripheral lymphoid tissues.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometric analysis of normal human peripheral blood cells. Use 5 µL (0.2 µg) per 100 µL cell sample (10⁵–10⁸ cells/test).

Compatibility Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for best results.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):

• Required for optimal staining with NovaFluor conjugates.
• Add 5 µL per sample before combining antibodies with cells.
• For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures optimized for minimal spillover and spread.

Spectral Properties:

• Excitation: 509 nm
• Emission: 665 nm
• Laser: 488 nm (Blue)

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Target Information

Human CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor of the selectin family. It includes:

• C-type lectin domain
• EGF-like domain
• Two SCR sequences
• Transmembrane and cytoplasmic regions

CD62L is constitutively expressed on most leukocytes including lymphocytes, monocytes, and polymorphonuclear cells, except a subset of memory T cells.

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For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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