Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), NovaFluor Blue 690, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.2 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532
• APC, APC-eFluor 780
• Brilliant UV 496, 615, 737, 805
• Brilliant Violet 421, 480, 650, 711, 786
• eFluor 450, FITC, Functional Grade
• NovaFluor Blue 510, 555, 585, 610-30S, 660-40S, 760
• NovaFluor Red 710, 725, 755
• NovaFluor Yellow 570, 590, 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600
• Request custom conjugation available

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Product Specific Information

Description:
The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells. It binds to glycosylated, fucosylated, and sulfated sialylated glycoproteins such as CD34, glycam-1, and MAdCAM-1, mediating lymphocyte rolling on activated endothelium and homing to high endothelial venules (HEV) of peripheral lymphoid tissues.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• Recommended use: 5 µL (1 µg) per test
• A test = amount of antibody staining a cell sample in 100 µL final volume
• Cell number: 10⁵–10⁸ cells/test (empirically determined)

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL of CellBlox Plus per sample before combining with cells.
• For single-color controls: add 5 µL per 100 µL of cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescent signatures and reduced spillover/spread.

Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

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Target Information

Human CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor. It contains:

• C-type lectin binding domain
• EGF-like domain
• Two short consensus repeat (SCR) sequences
• Spacer, transmembrane, and cytoplasmic regions

CD62L is constitutively expressed on most leukocytes, including lymphocytes, monocytes, and polymorphonuclear cells, except for many memory T cells.

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For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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