Thermo Fisher Scientific CD45 Monoclonal Antibody (IBL-5/25)
Thermo Fisher Scientific의 CD45 단일클론 항체(IBL-5/25)는 마우스 시료에서 CD45 검출용으로 사용됩니다. IHC, Flow Cytometry, IP 등에 적합하며, Rat IgG1 형식의 비결합 항체입니다. Protein G 정제, PBS 버퍼, 4°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Flow Cytometry (Flow) | 3–5 µg/1×10⁶ cells | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IBL-5/25 |
| Immunogen | Mouse lymph node cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1072403 |
Available Conjugated Formats
Product Specific Information
- MA1-70096 detects CD45 in mouse samples.
- Successfully used in flow cytometry, immunohistochemistry (frozen sections), and immunoprecipitation.
- Immunogen: mouse lymph node cells.
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) expressed in all nucleated hematopoietic cells, comprising about 10% of lymphocyte surface proteins. It is absent in non-hematopoietic tissues and cell lines. CD45 regulates Src-family kinases, is essential in lymphocyte development and antigen receptor signaling, and modulates cytokine receptor pathways.
Different isoforms (e.g., CD45RA) arise from alternative splicing and vary in expression depending on cell type and physiological state. CD45 also influences integrin-mediated signaling, apoptosis, and immune regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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