Thermo Fisher Scientific Calcineurin B homologous protein 2/HCC Antigen 520 Monoclonal Antibody (CPTC-CHP2-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

1-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2c, kappa

Class

Monoclonal

Type

Antibody

Clone

CPTC-CHP2-1

Immunogen

Recombinant human full-length CHP2 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CHP2,uniProtId:O43745-1,ncbiNodeId:9606,antigenRange:1-196,antigenLength:196,antigenImageFileName:63928-MSM1-P0_CHP2_O43745-1_House_mouse.svg,antigenImageFileNamePDP:63928-MSM1-P0_CHP2_O43745-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

CHP2 (Calcineurin B homologous protein 2), also known as Hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains four EF-hand domains and plays a potential role in transmembrane Na+/H+ exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.