Thermo Fisher Scientific IL-33R (ST2) Monoclonal Antibody (RMST2-2), Super Bright 600, eBioscience
IL-33R(ST2) 단일클론 항체 RMST2-2는 Mouse 시료에 반응하며 Super Bright 600 형광체로 결합된 Flow Cytometry용 항체입니다. IL-33/ST2 신호전달 연구에 적합하며, 높은 특이성과 안정성을 제공합니다. 4°C 암조건 보관, 동결 금지.
- 카탈로그번호
- 63-9335-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-33R (ST2) Monoclonal Antibody (RMST2-2), Super Bright 600, eBioscience
Thermo Fisher Scientific IL-33R (ST2) Monoclonal Antibody (RMST2-2), Super Bright 600, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 600 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RMST2-2 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2717063 |
Available Formats
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 615
- Brilliant UV 737
- Brilliant Violet 421
- eFluor 450
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 645
- Custom conjugation available
Product Specific Information
The RMST2-2 monoclonal antibody reacts with ST2 (IL-33 receptor, IL-33R), a member of the IL-1 receptor family. ST2 forms a ligand-dependent dimer with IL-1R accessory protein, and the IL-33/ST2 interaction induces TH2 cytokine production.
ST2 has both membrane-bound and soluble isoforms; the soluble form acts as a decoy receptor blocking IL-33/ST2 interactions. It is expressed by TH2 lymphocytes, mast cells, eosinophils, basophils, innate lymphocytes, smooth muscle cells, and endothelial cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow-derived mast cells at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Use a 610/20 bandpass filter and ensure instrument compatibility.
Using multiple Super Bright dyes: Use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions.
Light sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).
Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
IL-33R (ST2) is a receptor for IL-33 and a member of the IL-1 receptor family. It contains three Ig-like extracellular domains interacting with IL-1RAcP and IL-33. IL-33 binding activates NF-κB and MAPK pathways via adaptor proteins (MyD88, IRAK1, IRAK4, TRAF6), leading to cytokine release (IL-5, IL-6, IL-8, IL-13).
Two isoforms exist: ST2L (membrane-bound) and ST2S (soluble). The soluble form acts as a decoy receptor. IL-33R is expressed mainly on mast cells, basophils, eosinophils, and in mouse on ILC2 and Th2 cells. It is implicated in allergy, arthritis, atherosclerosis, and sepsis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.