Thermo Fisher Scientific CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), Super Bright 600, eBioscience
인간 및 비인간 영장류 CD185(CXCR5)를 인식하는 MU5UBEE 단클론 항체로, Super Bright 600 형광체로 결합되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, B세포 및 여포성 보조 T세포 연구에 적합합니다. 광 민감성이 있으므로 어둡게 보관하십시오.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 6 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate, Rhesus monkey |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MU5UBEE |
| Immunogen | N-terminal extracellular domain of CXCR5 (UniProt ID: P32302-1, antigen length: 372 aa) |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2724065 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 532, 700
- APC, Biotin
- Brilliant UV/Violet series (395–805)
- eFluor 450, FITC
- NovaFluor Blue, Red, Violet, Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 645, 702
- Request custom conjugation
Product Specific Information
The MU5UBEE monoclonal antibody reacts with human and non-human primate CD185 (CXCR5/BLR1), a seven-transmembrane G protein-coupled receptor originally identified in Burkitt’s lymphoma.
In peripheral blood, CD185 is expressed on B cells, CD4+ T cells (excluding Th1/Th2), and certain memory T cells.
CD185+ T cells migrate to lymph nodes due to CCR7 and CD62L expression and support B cell antibody production in tonsils.
CXCR5 plays a crucial role in lymphocyte trafficking, particularly T cell migration into B cell follicles in response to CXCL13, marking follicular helper T cells.
Applications Reported: Flow cytometry
Applications Tested: Pre-diluted and tested for flow cytometric analysis at 5 µL (0.5 µg)/test.
Excitation: 405 nm; Emission: 600 nm; Laser: Violet
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Use a 610/20 bandpass filter.
When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.
Light Sensitivity: Protect from light.
Fixation: Samples may be stored up to 3 days at 4°C in the dark using
- IC Fixation Buffer (Product # 00-8222)
- 1-step Fix/Lyse Solution (Product # 00-5333)
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Target: CXCR5 (C-X-C Chemokine Receptor Type 5, CD185, BLR1)
CXCR5 is a G protein-coupled receptor for chemokine BLC, expressed in Burkitt’s lymphoma and lymphoid tissues such as lymph node follicles and spleen.
It plays an essential role in B cell migration and is expressed in human blood, salivary gland, and various animal tissues (lymph node, spleen, liver, brain, etc.).
CXCR5 belongs to the GPCR family, which shares a seven-helix transmembrane structure and is a major pharmacological target in signaling research.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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