Thermo Fisher Scientific LYVE-1 Human ProcartaPlex Simplex Kit

Invitrogen™ LYVE-1 Human ProcartaPlex™ Simplex Kit

The Human LYVE-1 Simplex ProcartaPlex Kit measures LYVE-1 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel utilizing Luminex xMAP technology for protein detection and quantitation. When combining multiple Simplex kits (i.e., when not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed per assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.

• More results per sample: Measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology: Highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures identified by Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, MAGPIX). Similar to a sandwich ELISA, matched antibody pairs identify the protein of interest. Each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are detected using biotinylated antibodies and streptavidin–R-phycoerythrin (RPE).

The key difference from a traditional ELISA is that the capture antibody in ProcartaPlex assays is conjugated to a bead, not adsorbed to the microplate well, allowing reagents to remain free-floating in solution. Detection uses dual lasers—one to read bead signatures and another to quantify RPE fluorescence proportional to protein concentration. This enables profiling of more targets using less sample and time compared to conventional ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species: human, mouse, rat, nonhuman primate, porcine, and canine.

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