Thermo Fisher Scientific TREM-1 Human ProcartaPlex Simplex Kit
TREM-1 단백질을 정량 측정하는 휴먼 ProcartaPlex™ Simplex Kit로, Luminex xMAP 기술을 활용하여 다중 단백질 분석이 가능합니다. 25–50 μL의 샘플로 빠르고 정확한 결과를 얻을 수 있으며, 다른 Simplex 키트와 조합하여 맞춤형 멀티플렉스 패널 구성이 가능합니다.
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Invitrogen™ TREM-1 Human ProcartaPlex™ Simplex Kit
The Human TREM-1 Simplex ProcartaPlex Kit measures TREM-1 protein and is designed to be combinable with other Simplex kits to create custom multiplex panels utilizing Luminex xMAP technology for protein detection and quantitation. When combining multiple Simplex kits, only one buffer kit (sold separately) is needed per assay plate regardless of plex size.
About ProcartaPlex Assays for the Luminex Platform
ProcartaPlex immunoassays are based on the sandwich ELISA principle, using two highly specific antibodies binding to different epitopes of a protein to quantitate multiple targets simultaneously using a Luminex instrument.
These assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and deliver results within four hours.
Key Features
- Multiplex capability: Measure multiple protein targets simultaneously in a single 25–50 μL sample
- Reliable Luminex technology: Well-established and highly referenced multiplexing platform for protein detection and quantitation
- High efficiency: Profile more targets using less sample and time compared to traditional ELISA
Technology Overview
ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for simultaneous detection and quantitation of up to 80 protein targets in a single small-volume sample.
Each Luminex bead is internally dyed with unique ratios of red and infrared fluorophores, creating distinct spectral signatures identifiable by Luminex detection systems (e.g., Luminex 200, FLEXMAP 3D, MAGPIX).
In the assay:
- Each bead is conjugated with an antibody specific to a single target protein.
- Bound proteins are detected using biotinylated antibodies and streptavidin–R-phycoerythrin (RPE).
- The Luminex instrument uses lasers to identify bead signatures and quantify fluorescence intensity proportional to protein concentration.
Unlike traditional ELISA, the capture antibody in the ProcartaPlex assay is bead-conjugated and free-floating in solution, allowing for higher multiplexing capability and reduced sample consumption.
ProcartaPlex multiplex panels are available for six species: human, mouse, rat, nonhuman primate, porcine, and canine.
제품 사양
| 항목 | 내용 |
|---|---|
| 제품 유형 | Simplex Kit |
| 수량 | 96 Tests |
| 종 | Human |
| Combinability | Combinable |
| 비드 유형 | TREM-1 [14] |
| 어세이 민감성 | Lot 1 기준: 139.3 pg/mL |
| 어세이 범위 | Lot 1 기준: 689–2,820,100 pg/mL |
| 형식 | Simplex Kit |
| 사용 장비 | Luminex™ Instruments |
| 유전자 | Triggering receptor expressed on myeloid cells 1 |
| 유전자 별칭 | CD354; TREM-1 |
| 유전자 ID (Entrez) | 54210 |
| 유전자 기호 | TREM1 |
| 제품 라인 | ProcartaPlex™ |
| 단백질 | Triggering receptor expressed on myeloid cells 1 |
| 단백질 아형 | Triggering receptor expressed on monocytes 1, CD354 |
| 샘플 종류 | Serum, Plasma, Cell Culture Supernatants |
| 샘플 부피 | Serum/Plasma: 25 μL, Cell Culture Supernatant: 50 μL |
| UniProt ID | Q9NP99 |
| 배송 조건 | Wet Ice |
| 연구 분야 | Immunology |
| Unit Size | Each |
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