Thermo Fisher Scientific CD24 Monoclonal Antibody (30-F1), PE, eBioscience
마우스 CD24 항원을 인식하는 단클론 항체로, PE 형광체가 결합되어 있습니다. Flow Cytometry에 적합하며, 0.125 µg/test로 사용 가능합니다. 488–561 nm에서 여기, 578 nm에서 방출됩니다. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.125 µg/test
- Publications: View 17 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2c, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 30-F1 |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465599 |
Additional Formats
- Brilliant UV 805
- FITC
- Custom conjugation available upon request
Product Specific Information
The 30-F1 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35–50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage. CD24 expression detected by 30-F1 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.
CD24 is a variably glycosylated molecule, resulting in heterogeneity of molecular mass across different lineages. Different antibodies to CD24 may show subtle differences in staining intensity on lymphocyte populations.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse splenocytes and bone marrow cells
- Recommended ≤ 0.125 µg/test
- A test is defined as the amount of antibody staining a 100 µL sample (10⁵–10⁸ cells/test)
- Antibody titration recommended for optimal performance
Optical Properties:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, from pro-B cells in bone marrow to mature B cells. Plasma cells show low or negative expression. CD24 is also expressed in most B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas. It may regulate B-cell proliferation, maturation, and autoimmunity control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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