Thermo Fisher Scientific CD24 Monoclonal Antibody (30-F1), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.125 µg/test
• Publications: View 17 publications

Product Specifications

Additional Formats

• Brilliant UV 805
• FITC
• Custom conjugation available upon request

Product Specific Information

The 30-F1 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35–50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage. CD24 expression detected by 30-F1 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.

CD24 is a variably glycosylated molecule, resulting in heterogeneity of molecular mass across different lineages. Different antibodies to CD24 may show subtle differences in staining intensity on lymphocyte populations.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes and bone marrow cells
  • Recommended ≤ 0.125 µg/test
  • A test is defined as the amount of antibody staining a 100 µL sample (10⁵–10⁸ cells/test)
  • Antibody titration recommended for optimal performance

Optical Properties:

• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, from pro-B cells in bone marrow to mature B cells. Plasma cells show low or negative expression. CD24 is also expressed in most B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas. It may regulate B-cell proliferation, maturation, and autoimmunity control.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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