Thermo Fisher Scientific DAPK2 Monoclonal Antibody (OTI1B5), TrueMAB
DAPK2 단백질을 인식하는 Mouse IgG2b 단일클론 항체로, Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합함. 인간 시료에 반응하며, 동결건조 형태로 제공. 재구성 후 약 1 mg/mL 농도로 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B5 |
| Immunogen | Full-length human recombinant DAPK2 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
DAPK2 is a calcium/calmodulin-dependent kinase that acts as a positive regulator of apoptosis and is expressed in many tissue types. It belongs to the serine/threonine protein kinase family and contains an N-terminal kinase domain followed by a conserved calmodulin-binding domain similar to DAPK1. Overexpression of DAPK2 induces apoptosis and utilizes multiple polyadenylation sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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