Thermo Fisher Scientific NIT1 Monoclonal Antibody (OTI3A11), TrueMAB
인간 NIT1 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC/IF, 유세포분석 등에 사용 가능. 종 특이성은 인간이며, 동결건조 형태로 제공. 세포 성장 및 암세포의 아폽토시스 반응 연구에 적합.
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Thermo Fisher Scientific NIT1 Monoclonal Antibody (OTI3A11), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A11 |
| Immunogen | Full length human recombinant protein of human NIT1 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended desalting product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
NIT1 plays a role in cell growth and apoptosis; loss of expression promotes cell proliferation and resistance to DNA damage stress.
It exhibits tumor suppressor properties and enhances apoptotic responsiveness in cancer cells, acting additively with FHIT.
NIT1 negatively regulates primary T-cell activation and lacks omega-amidase activity like NIT2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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