Thermo Fisher Scientific CD163 Monoclonal Antibody (OTI2C7), TrueMAB

Applications

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein belonging to the scavenger receptor cysteine-rich superfamily. It acts as a receptor for the hemoglobin-haptoglobin complex, protecting tissues from oxidative damage and facilitating iron recycling via endocytosis.

CD163 is expressed on human monocytes and macrophages, particularly in the late phase of inflammation. It is present on all circulating monocytes and most tissue macrophages, except those in mantle zones and germinal centers. Expression is upregulated by IL-10, IL-6, and dexamethasone, while LPS and PMA induce shedding of CD163 into plasma or supernatant.

Binding of hemoglobin/haptoglobin complexes by CD163 is calcium- and pH-dependent, with higher affinity for multimeric haptoglobin (HP1F phenotype). CD163 also triggers intracellular signaling cascades involving tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and secretion of IL-6 and CSF1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.