Thermo Fisher Scientific CD177 Monoclonal Antibody (MEM-166), Alexa Fluor 647
CD177 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Alexa Fluor 647 형광 표지. Flow Cytometry 및 Western Blot에 사용 가능. 인간 및 비인간 영장류 반응성. PBS/BSA buffer로 보관하며, 4°C 암소 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Flow Cytometry (Flow) | 4 µL / 100 µL whole blood or 10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-166 |
| Immunogen | Human granulocytes |
| Conjugate | Alexa Fluor™ 647 |
| Excitation / Emission Max | 650 / 671 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539503 |
Available Formats
- Unconjugated (MA1-19250)
- APC (MA1-10119)
- FITC (MA1-19597)
- PE (MA1-10120)
- Custom conjugation available upon request
Product Specific Information
This antibody reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of the uPAR family.
It is expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes.
Target Information
CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein mainly expressed on neutrophils.
Its expression increases during pregnancy, inflammation, or after G-CSF application.
CD177 interacts with CD31 (PECAM-1) and is involved in neutrophil transmigration and proliferation.
Antibodies against CD177 can be implicated in neonatal alloimmune neutropenia (NAN).
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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