Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), Alexa Fluor 647

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

4 µLx100 µL whole blood or 10^6 cells

-

Product Specifications

Species Reactivity

Human, Non-human primate

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3G8

Immunogen

Human neutrophils if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD16,uniProtId:O75015-1,ncbiNodeId:9606,antigenRange:1-233,antigenLength:233,antigenImageFileName:MA5-18128_CD16_O75015-1_House_mouse.svg,antigenImageFileNamePDP:MA5-18128_CD16_O75015-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

View additional formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-Cyanine7
• Biotin
• DyLight 594
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP
• PerCP-Cyanine5.5
• Request custom conjugation

Excitation/Emission Max

650/671 nm View spectra

Form

Liquid

Purification

Size-exclusion chromatography

Storage buffer

PBS with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539502

Product Specific Information

This antibody detects both 158V and 158F allotype on natural NK cells.

Target Information

CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.