![Thermo Fisher Scientific CD34 Monoclonal Antibody (4H11[APG]), Alexa Fluor 488](https://images.cacheby.com/product-images/cacheby/57056430-28b8-11f0-bf4f-b7c32b12ae43/primary-antibody-image.jpg-650.jpg)
Thermo Fisher Scientific CD34 Monoclonal Antibody (4H11[APG]), Alexa Fluor 488
CD34 단백질을 인식하는 mouse IgG1 단클론 항체로, Alexa Fluor 488 형광 표지. 인간 hematopoietic stem cell 및 progenitor cell 연구용. Flow cytometry, ICC/IF, WB 등 다양한 응용에 적합. 4°C 암소 보관, 동결 금지.
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Applications
Western Blot (WB)
- Assay-dependent
Immunocytochemistry (ICC/IF)
- Assay-dependent
Flow Cytometry (Flow)
- 4 µL/100 µL whole blood or 10^6 cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4H11[APG] |
| Immunogen | Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539510 |
Additional Formats
- Unconjugated (MA1-19229)
- APC (MA1-19466)
- Biotin (MA1-19515)
- FITC (MA1-19593)
- PE (MA1-19645)
- Request custom conjugation available
Product Specific Information
This antibody reacts with extracellular class III epitope on CD34, a 110–115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitor cells and pluripotential stem cells. The antibody 4H11[APG] completely blocks binding of class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
Target Information
CD34 is a highly glycosylated monomeric surface protein (111–115 kDa) present on various stem cell populations. It serves as a stem cell marker and may function as a receptor involved in adhesion, differentiation, and proliferation of hematopoietic progenitors. CD34 expression indicates a specific developmental state and may facilitate cell-matrix interactions in bone marrow. It also acts as a scaffold for glycans, enabling binding to lectins and selectins. The intracellular domain is phosphorylated by protein kinase C, suggesting a role in signal transduction. CD34 dysfunction is associated with dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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