Thermo Fisher Scientific Phospho-DCLK1 (Ser30) Polyclonal Antibody
Phospho-DCLK1 (Ser30) 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC(P) 분석에 적합합니다. 사람, 마우스, 랫트 등 다양한 종에 반응하며 항원 친화 크로마토그래피로 정제되었습니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to N-terminus of rat DCLK1 at the phosphorylation site of DCLK1 (S30(b)/337(a)) at Serine 30. Covers epitope of -SPSPT-S-PGSLR-. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Human brain tissue
- Cellular location: Cytoplasm
Target Information
DCAMKL1 (DCLK1, doublecortin-like kinase 1) is a serine/threonine kinase containing two N-terminal doublecortin domains that bind microtubules and regulate their polymerization. It also includes a C-terminal serine/threonine protein kinase domain homologous to Ca2+/calmodulin-dependent kinase and a central serine/proline-rich region mediating multiple protein-protein interactions.
DCLK1 can undergo autophosphorylation and exhibits microtubule-polymerizing activity independent of its kinase function. It is involved in neuronal migration, retrograde transport, apoptosis, and neurogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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