Thermo Fisher Scientific SirT1 Polyclonal Antibody
SirT1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC(P) 실험에 적합. Human, Mouse, Rat에 반응하며, 항원 친화 크로마토그래피로 정제됨. 세포 내 SIRT1 연구 및 대사, 자가포식, 세포주기 관련 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide NEAISVKQEVTDMNYPSNKS corresponding to human SirT-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Colon
- Cellular location: Cytoplasm, mitochondria, nucleus
Target Information
NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis, and autophagy.
SIRT1 modulates chromatin function through deacetylation of histones and promotes alterations in histone and DNA methylation, leading to transcriptional repression.
It plays an essential role in skeletal muscle cell differentiation and mediates the inhibitory effect of low nutrients on myoblast differentiation, involving AMPK and NAMPT pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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