Thermo Fisher Scientific FOXP3 Recombinant Rabbit Monoclonal Antibody (6K3D9)
FOXP3 단백질을 인식하는 Thermo Fisher의 재조합 Rabbit Monoclonal 항체(6K3D9). Western blot, ELISA, IP 등 다양한 응용 가능. 인간 FOXP3(aa 286–431) 기반 면역원 사용. 고순도 Affinity Chromatography 정제, 안정적 액상 형태로 제공.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 6K3D9 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 286–431 of human FOXP3 (Q9BZS1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.36 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2912008 |
Product Specific Information
- Positive test controls: Jurkat cells
- Localization: Cytoplasm, Nucleus
- Immunogen sequence:
GSQGPVVPAW SGPREAPDSL FAVRRHLWGS HGNSTFPEFL HNMDYFKFHN MRPPFTYATL IRWAILEAPE KQRTLNEIYH WFTRMFAFFR NHPATWKNAI RHNLSLHKCF VRVESEKGAV WTVDELEFRK KRSQRPSRCS NPTPGP
Target Information
FOXP3 (Forkhead box protein 3) is a transcriptional regulator belonging to the forkhead/winged-helix family, essential for immune homeostasis. It is 381 amino acids long and expressed at high levels in CD25⁺ CD4⁺ regulatory T cells, lower in CD4⁺/CD25⁻ cells, and absent in CD4⁻/CD8⁺ T cells. FOXP3 acts as a master regulatory gene and a specific marker for regulatory T cells. Mutations in FOXP3 cause the scurfy phenotype in mice and IPEX syndrome in humans. Expression of FOXP3 in human breast and colon cancer cells is regulated by p53 in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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