Thermo Fisher Scientific ErbB2 (HER-2) Monoclonal Antibody (MJD2), eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

This MJD2 monoclonal antibody reacts with HER-2, the product of the ErbB2 gene and a member of the human epidermal growth factor receptor (EGFR) family. HER-2 is a 185 kDa transmembrane receptor with extracellular and intracellular tyrosine kinase domains. Activation occurs via homodimer or heterodimer formation with EGFR family members (HER3, HER4).

Overexpression of HER-2 leads to auto-phosphorylation, increased proliferation, and invasiveness, and is associated with breast, ovarian, cervical, uterine, and gastric cancers. HER-2 targeted agents are widely used for HER-2-positive breast cancer and may also inhibit proliferation of breast cancer stem cells in HER-2-negative patients, highlighting HER-2’s broad research relevance.

The MJD2 antibody recognizes the extracellular domain of HER-2 at an epitope distinct from those recognized by 4D5 (Herceptin) and SP3 monoclonal antibodies.

Applications Reported

• Western blotting
• Immunohistochemistry (formalin-fixed paraffin-embedded tissues)
• Microscopy
• Immunocytochemistry

Applications Tested

• IHC on formalin-fixed paraffin-embedded human tissue: ≤ 5 µg/mL
• ICC on methanol-fixed and permeabilized human BT474 cells: ≤ 5 µg/mL
• WB on reduced human BT474 cell lysate: ≤ 5 µg/mL
  It is recommended to titrate the antibody for optimal performance.

Target Information

ErbB2 (HER2) is a receptor tyrosine kinase overexpressed in certain breast tumors. Herceptin, a monoclonal antibody targeting HER2, is used in treatment of metastatic HER2-positive cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.