Thermo Fisher Scientific CD5 Monoclonal Antibody (L17F12), APC, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.03 µg)/test

Product Specifications

Additional Formats

• FITC
• PE-Cyanine5
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The L17F12 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by most thymocytes, mature T cells, and a subset of B cells. CD5 signaling activates T cells and mediates T-B interactions through binding to CD72 on B cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested by flow cytometry on normal human peripheral blood cells
  • Recommended use: 5 µL (0.03 µg) per test
  • Test definition: Antibody amount sufficient for staining a 100 µL cell sample
  • Cell number: 10⁵–10⁸ cells/test (empirically determined)
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD5 is a 67 kDa single-chain transmembrane glycoprotein found on all mature T-lymphocytes, most thymocytes, and many T-cell leukemias and lymphomas. It also appears on a subset of activated B-cells and may regulate T-cell proliferation. CD5 is expressed on approximately 95% of thymocytes and 72% of peripheral blood lymphocytes, with strong reactivity in T-cell areas of lymph nodes. Aberrant CD5 expression is associated with thymus cancer and Richter’s Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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