Thermo Fisher Scientific Mouse anti-Rat IgG2a Secondary Antibody, eFluor 660, eBioscience

Applications

Tested Dilution

• Flow Cytometry (Flow): 0.25 µg/test

Product Specifications

Additional Formats

• eFluor 570
• eFluor 615
• FITC
• PE
• PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a secondary reagent in flow cytometry and microscopy.
This antibody does not recognize other rat isotypes and shows no cross-reactivity with mouse IgG2a or other mouse isotypes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometric analysis of mouse splenocytes and human peripheral blood cells stained with Rat IgG2a primary antibody
• Recommended Use: ≤0.25 µg/test (in 100 µL final volume; 10⁵–10⁸ cells/test)
• Optimization: Titrate for best performance in your assay

eFluor® 660 is a replacement for Alexa Fluor® 647, emitting at 659 nm and excited by a red laser (633 nm).
Ensure your instrument supports detection of this fluorochrome.

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Anti-Rat secondary antibodies are affinity-purified with well-characterized specificity for rat immunoglobulins.
They are useful for detection, sorting, or purification of target antibodies and provide signal amplification as multiple secondaries can bind a single primary antibody.
These antibodies are typically generated by immunizing the host with pooled rat immunoglobulins and may be further purified or conjugated for enhanced specificity and performance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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