Thermo Fisher Scientific Mouse anti-Rat IgG2b Secondary Antibody, eFluor 660, eBioscience

Thermo Fisher Scientific Mouse anti-Rat IgG2b Secondary Antibody, eFluor 660, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• Biotin
• eFluor 570
• FITC
• PE
• Custom conjugation available upon request

Product Specific Information

The monoclonal antibody R2B-7C3 recognizes Rat IgG2b antibodies and can be used as a second-step reagent in flow cytometry and microscopy. It does not cross-react with other rat isotypes or mouse antibodies.

Applications Reported: Flow cytometry, immunohistochemistry (frozen sections), microscopy, and immunocytochemistry.

Applications Tested:

• In IHC(F) on frozen mouse spleen with Rat IgG2b primary antibody: ≤ 20 µg/mL
• In flow cytometry with Rat IgG2b primary antibody: ≤ 0.06 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)

Careful titration is recommended for optimal performance.

Fluorophore Information:
eFluor® 660 is an alternative to Alexa Fluor® 647.

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Anti-Rat secondary antibodies are affinity-purified reagents with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of target molecules. Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.

Common production methods include immunization with pooled rat immunoglobulins, followed by purification and conjugation (e.g., immunoaffinity chromatography, fragmentation, label conjugation).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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