Thermo Fisher Scientific FOXP3 Monoclonal Antibody (236A/E7), eFluor 660, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC
• Biotin
• eFluor 450
• eFluor 570
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• Custom conjugation available

Product Specific Information

The 236A/E7 antibody reacts with human Foxp3 (FORKHEAD BOX P3, SCURFIN, JM2), a 49–55 kDa transcriptional regulator. Foxp3 is highly expressed in CD4+CD25+ regulatory T cells (Treg) and can induce a Treg phenotype when ectopically expressed in CD4+CD25− cells.

Intracellular staining and flow cytometric analysis of human PBMCs using this antibody and the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523) show staining of the CD4+CD25^bright population.

The 236A/E7 epitope is distinct from that of clone PCH101. This antibody also recognizes rhesus macaque, sooty mangabey, and cynomolgus macaque.

Applications Reported: Intracellular staining and flow cytometric analysis.
Applications Tested: Pre-titrated and validated with the Foxp3/Transcription Factor Staining Buffer Set. Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:
eFluor® 660 replaces Alexa Fluor® 647, emits at 659 nm, and is excited by a 633 nm red laser.
Excitation: 633–647 nm; Emission: 668 nm; Laser: Red.
Filtered post-manufacture (0.2 µm).

Target Information

FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis. It is expressed at high levels in CD25+CD4+ regulatory T cells, low levels in CD4+CD25− cells, and absent in CD4−CD8+ T cells. FOXP3 acts as a master regulatory gene and marker for Tregs. Mutations cause the scurfy phenotype in mice and IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked) in humans. FOXP3 expression in human breast and colon cancer cells is regulated by p53 in response to DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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