Thermo Fisher Scientific Phosphotyrosine Recombinant Rabbit Monoclonal Antibody (RM111)
Phosphotyrosine 특이적 recombinant rabbit monoclonal antibody (Clone RM111). Western blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. 높은 특이성과 로트 간 일관성 제공. 연구용으로 신호전달 및 단백질 인산화 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 1:200–1:500 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 1:200–1:1,000 |
| ChIP assay (ChIP) | 1:200–1:1,000 |
| Luminex (LUM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM111 |
| Immunogen | Phosphotyrosine-BSA conjugate |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744992 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Tyrosine phosphorylation plays a key role in mitogenic signal transduction from transmembrane receptors and in transformation by oncogene tyrosine kinases. Phosphorylation of specific tyrosine residues is a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression, and oncogenic transformation.
Antibodies recognizing phosphorylated tyrosine residues are invaluable tools for studying tyrosine-phosphorylated proteins and their biochemical pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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