Thermo Fisher Scientific Phospho-SRC (Tyr418) Monoclonal Antibody (SC1T2M3), eBioscience
인산화된 SRC(Tyr418)을 인식하는 마우스 단클론 항체로, 인간 및 마우스 시료에 반응합니다. Western blot, IHC, ICC 등 다양한 응용에 적합하며, 높은 순도(>90%)와 안정적인 액상 형태로 제공됩니다. 세포 신호전달 및 암 연구용으로 최적화된 연구용 항체입니다.
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Thermo Fisher Scientific Phospho-SRC (Tyr418) Monoclonal Antibody (SC1T2M3), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SC1T2M3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865321 |
Additional Formats Available:
- eFluor 660
- PE
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
This SC1T2M3 monoclonal antibody recognizes human and mouse Src tyrosine kinase (also known as ASV, c-src, c-SRC, p60-Src, pp60c-src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, SRC1) when phosphorylated on tyrosine 418 (Y418).
Autophosphorylation of Src at Y418 in the catalytic domain is required for full catalytic activity of this kinase. Src is a non-receptor tyrosine kinase involved in signal transduction in numerous biological systems and is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins, receptor tyrosine kinases, GPCRs, and cytokine receptors.
Aberrant Src activity has been implicated in various cancers. Due to sequence homology around Src Y418, this SC1T2M3 clone is predicted to cross-react with Src family kinases including Src, Lck, Fyn, and Lyn.
Specificity Determination:
Confirmed by ELISA, flow cytometry, and western blotting.
Applications Reported:
- Western blotting (use ≤5 µg/mL)
- Fluorochrome-conjugated SC1T2M3 recommended for intracellular flow cytometry
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in embryonic development and cell growth regulation. It associates with various cell surface receptors to facilitate signal transduction and plays roles in cytoskeletal organization, cell cycle regulation, and apoptosis.
In humans, SRC is located on chromosome 20. Activity is regulated by phosphorylation at Tyr416 (activation) and Tyr527 (inhibition). Mutations may contribute to malignant progression of colon cancer. Two transcript variants encoding the same SRC protein are known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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