Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), PE, eBioscience
인간 PD-L1(B7-H1)을 인식하는 MIH1 클론 단클론항체로, PE 형광표지되어 Flow Cytometry에 최적화됨. 565/576 nm에서 여기/방출. 4°C 암소 보관, 동결 금지. 인간 세포 분석 및 종양 면역 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 63 publications |
| Functional Assay (Functional) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MIH1 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11042721 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 786
- eFluor 450
- Functional Grade
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Yellow 700
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
Description:
The MIH1 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. It is expressed on a majority of leukocytes and serves as a ligand for PD-1. Interaction of PD-1 with PD-L1 (B7-H1) or PD-L2 (B7-DC) inhibits T and B cell responses. MIH1 is reported to be a blocking antibody.
Applications Reported:
The MIH1 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
This antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.5 µg) per test. One test is defined as the amount of antibody that stains a cell sample in a final volume of 100 µL. Cell number may vary from 10⁵ to 10⁸ cells/test.
Fluorescence Information:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a B7 family member that binds to the programmed death receptor 1 (PD-1). This interaction negatively regulates T-cell receptor (TCR) signaling, decreasing TCR-mediated proliferation and cytokine production. PD-L1 is abundantly expressed on many murine and human cancers and can be upregulated upon IFN-gamma stimulation, suggesting its role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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