Thermo Fisher Scientific CD370 (Clec9A) Monoclonal Antibody (42D2), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

42D2

Conjugate

PE PE PE

View additional formats

• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10596644

Product Specific Information

Description: This 42D2 monoclonal antibody reacts with mouse CD370, which is also known as DNGR-1 or Clec9A. CD370 is a C-type lectin-like receptor expressed on conventional and plasmacytoid dendritic cells (DCs). In particular, CD8+ conventional DCs in the thymus, spleen, and lymph nodes, as well as CD24+ DCs in the blood, express CD370. This receptor is not detectable on most B cells, T and NK cells, monocytes, and macrophages. The cytoplasmic domain of CD37° Contains an ITAM motif that has been demonstrated to phosphorylate Syk tyrosine kinase, leading to NFAT activation. This signaling reportedly mediates the presentation of necrotic cell-associated antigens by CD8+ DCs in innate immunity.

Applications Reported: This 42D2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 42D2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.