Thermo Fisher Scientific CD370 (Clec9A) Monoclonal Antibody (42D2), PE, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 1 µg/test
• View 4 publications

Product Specifications

Additional Formats

• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
This 42D2 monoclonal antibody reacts with mouse CD370 (also known as DNGR-1 or Clec9A), a C-type lectin-like receptor expressed on conventional and plasmacytoid dendritic cells (DCs). CD8⁺ conventional DCs in the thymus, spleen, and lymph nodes, as well as CD24⁺ DCs in the blood, express CD370. It is not detectable on most B cells, T and NK cells, monocytes, or macrophages.

The cytoplasmic domain of CD370 contains an ITAM motif that phosphorylates Syk tyrosine kinase, leading to NFAT activation. This signaling mediates the presentation of necrotic cell-associated antigens by CD8⁺ DCs in innate immunity.

Applications Reported:
This 42D2 antibody is reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤1 µg per test (defined as 100 µL staining volume). Optimal antibody titration is advised for best assay performance.

Excitation / Emission: 488–561 nm / 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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