Thermo Fisher Scientific MAP2K4 Monoclonal Antibody (OTI8A8), TrueMAB
MAP2K4 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC, Flow cytometry, IP 등 다양한 응용에 적합. Human 및 Dog 시료 반응. 동결건조 형태로 공급되며, 재구성 후 약 1 mg/mL 농도 유지. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8A8 |
| Immunogen | Full-length protein expressed in 293T cells transfected with human MAP2K4 expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K4 (MEK4), also known as mitogen-activated protein kinase 4, is a dual specificity kinase of the STE7 family that phosphorylates and activates JNK1/2 and p38. It preferentially phosphorylates tyrosine residues of JNKs and is involved in cellular stress and inflammatory responses.
MAP2K4 plays a role in carcinogenesis; loss-of-function mutations have been identified in lung and pancreatic tumors, while pro-oncogenic roles have also been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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