Thermo Fisher Scientific CHEK2 Monoclonal Antibody (OTI5C4), TrueMAB
CHEK2 단백질을 타겟으로 한 Mouse IgG1 단클론 항체로, Western blot, IHC, ICC, Flow cytometry, IP에 적합합니다. Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. DNA 손상 반응 및 암 관련 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 2 µg / 500 µL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5C4 |
| Immunogen | Full length human recombinant protein of human CHEK2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CHEK2 (CHK2) is a serine/threonine kinase involved in the DNA damage checkpoint pathway. Mutations in CHK2 are associated with various cancers. CHK2 phosphorylates several cell cycle regulators including tumor suppressor proteins such as BRCA1, aiding in DNA damage response and survival.
Mutations in CHK2 are linked to Li-Fraumeni syndrome and predispositions to sarcomas, breast cancer, and brain tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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