Thermo Fisher Scientific PLK1 Monoclonal Antibody (OTI6C6), TrueMAB
PLK1 단백질을 인식하는 Mouse Monoclonal Antibody (OTI6C6). Western blot, IHC, IP에 사용 가능. Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도. 세포 분열 및 암 연구용으로 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6C6 |
| Immunogen | Full length human recombinant protein of human PLK1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
PLK1 is a serine/threonine kinase involved in cell mitosis and serves as a marker of cell proliferation. It plays a key role in carcinogenesis.
Polo-like kinase proteins bind and phosphorylate proteins that are already phosphorylated on specific motifs recognized by POLO box domains.
PLK1 phosphorylates multiple substrates including BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, TP73, TPT1, and WEE1.
It is essential for centrosome function and bipolar spindle assembly through phosphorylation of KIZ, NEDD1, and NINL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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