Thermo Fisher Scientific EpCAM Monoclonal Antibody (VU-1D9), FITC
EpCAM 단백질을 인식하는 FITC 결합 단클론 항체로, 인간 유래 시료에서 사용 가능. Flow cytometry, IHC, ICC/IF 등에 적용. EGF-like domain I의 EpCAM 항원을 인식하며, 다양한 상피세포 및 암세포를 강하게 염색. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | – | 2 publications |
| Immunohistochemistry (IHC) | – | 3 publications |
| Immunocytochemistry (ICC/IF) | – | 1 publication |
| Flow Cytometry (Flow) | 20 µL / 1×10⁶ cells | 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VU-1D9 |
| Immunogen | Small cell lung carcinoma cell line H69 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11156192 |
Product Specific Information
This antibody recognizes an extracellular epitope within the EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. It strongly stains various normal epithelial cells and carcinomas.
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent cell adhesion molecule, influencing cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cyclins A/E. Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression correlates with enhanced proliferation and is common in human carcinomas, serving as a marker for epithelial tumors. Ep-CAM is expressed on the basolateral surface of most simple epithelia and many carcinoma types, and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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