Thermo Fisher Scientific EpCAM Monoclonal Antibody (VU-1D9), PE
EpCAM (CD326) 인식 단일클론 항체로, 인간 시료에서 IHC, ICC/IF, 유세포분석(Flow)에 사용 가능. PE 형광 표지로 565/576 nm에서 발광. EpCAM 발현 세포 및 상피계 암 연구에 적합. 4°C 암조건 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 20 µL / 1×10⁶ cells | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VU-1D9 |
| Immunogen | Small cell lung carcinoma cell line H69 |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154356 |
Additional Formats
Product Specific Information
This antibody recognizes an extracellular epitope within the EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. It strongly stains various normal epithelial cells and carcinomas.
Target Information
Ep-CAM (epithelial cell adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent homotypic adhesion molecule and influences cell cycle, proliferation, and metabolism by regulating proto-oncogene c-myc and cyclins A and E.
Ep-CAM overexpression is associated with enhanced epithelial proliferation and is a marker for tumors of epithelial lineage. It is expressed on the basolateral surface of most simple epithelia and many carcinomas, and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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