Thermo Fisher Scientific Human/Mouse/Rat BMP-2 Recombinant Protein, PeproTech
인간, 생쥐, 랫드 유래 BMP-2 재조합 단백질로 골형성 연구용. E. coli 발현, ≥98% 순도, 26 kDa 크기의 호모다이머. ATDC-5 세포에서 알칼리성 인산분해효소 유도 활성 검증. 상온 배송, -20°C 보관.
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Applications
Western Blot (WB)
- Assay-dependent
ELISA
- Assay-dependent
Functional Assay
- Assay-dependent
In vitro Assay (IV)
- Assay-dependent
Miscellaneous PubMed
- Assay-dependent
Immunostaining (IS)
- Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human, Mouse, Rat |
| Published Species | Camelid, Dog, Human, Mouse, Non-human primate, Pig, Rat, Sheep |
| Expression System | E. coli |
| Amino Acid Sequence | MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR |
| Molecular Weight | 26 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥ 98% by SDS-PAGE gel and HPLC analyses |
| Endotoxin Concentration | <1 EU/µg |
| Activity | Induces alkaline phosphatase production by ATDC-5 cells; ED50 = 0.5–1.0 µg/ml |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
Recombinant Human/Murine/Rat BMP-2 is a 26.0 kDa homodimeric protein consisting of two 115 amino acid polypeptide chains.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis.
Target Information
Bone Morphogenic Proteins (BMPs) are members of the TGF-beta superfamily that affect bone and cartilage formation (Hogan 1996, Reddi 1998, Francis-West et al. 1999).
Mature BMPs are 30–38 kDa proteins assuming a TGF-beta-like cysteine knot configuration. Lovostatin increases bone formation by activating the bmp-2 gene (Mundy et al. 1999).
BMPs stimulate bone matrix protein production and influence stromal cell and osteoclast proliferation (Macias et al. 1999, Lecanda et al. 1997).
They also play roles in visceral development, cell proliferation, apoptosis, differentiation, and morphogenesis (Hogan 1996, Dale and Wardle 1999).
BMPs are involved in dorsal/ventral patterning and signal through type II and type I receptors activating the Smad pathway (Massague 1994, Derynck 1997, Attisano 1993).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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