Thermo Fisher Scientific CD23 Monoclonal Antibody (EBVCS2), Super Bright 600, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.25 µg)/Test
• View 1 publication

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant UV 805
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 702
• Request custom conjugation

Product Specific Information

The EBVCS2 monoclonal antibody reacts with human CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells, and at low levels on T, NK, Langerhans cells, and platelets. Expression increases upon B cell activation, and soluble forms are biologically active. CD23 is a low-affinity receptor for IgE and plays a role in regulating IgE response and B cell activation. CD21 and the α-subunit of CD11b/CD11c bind to CD23.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. Use 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Super Bright 600:

• Excitation: 405 nm
• Emission: 600 nm
• Recommended filter: 610/20 bandpass
• Use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
• Light sensitive; protect from light.
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD23 is a 45 kDa glycoprotein found on subsets of peripheral blood and tonsil B cells, and strongly expressed on EBV-transformed B lymphoblasts. It functions as a low-affinity IgE receptor and is involved in B cell growth, differentiation, and IgE production. Soluble CD23 acts as a mitogenic factor. Abnormal CD23 expression is associated with chronic conjunctivitis and chronic lymphocytic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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