Thermo Fisher Scientific ApoA1 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MDEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human ApoA-I. Anti-Human ApoA-I-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human ApoA-I by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human ApoA-I (500-P331) as a capture antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human ApoA-I.

Western Blot:
To detect Human ApoA-I by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible development reagents, the detection limit for Recombinant Human ApoA-I is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P331BT-1MG will be provided as 2 × 500 µg.

Target Information

Apolipoprotein A-1 (ApoA-I) in human plasma is a major protein component of high-density lipoprotein (HDL). The molecular mass is approximately 24 kDa. ApoA-I acts as a cofactor for enzymes involved in cholesterol metabolism and is produced in the liver. Plasma concentration is typically 270–380 mg/100 mL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.