Thermo Fisher Scientific ApoA1 Polyclonal Antibody, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.5–2.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MDEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human ApoA-I. Anti-Human ApoA-I-specific antibody was purified by affinity chromatography employing an immobilized Human ApoA-I matrix.

Sandwich ELISA:
To detect Human ApoA-I by sandwich ELISA (using 100 µL/well), a concentration of 0.5–2.0 µg/mL of this antibody is required. When used with PeproTech Biotinylated Anti-Human ApoA-I (500-P331BT) as a detection antibody, allows detection of at least 2000–4000 pg/mL of Recombinant Human ApoA-I.

Western Blot:
To detect Human ApoA-I by Western blot analysis, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, detection limit for Recombinant Human ApoA-I is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Target Information

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high-density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 acts as a cofactor for enzymes involved in cholesterol turnover and is produced in the liver. Plasma concentration is 270–380 mg/100 mL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.