Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (14D3), Biotin, eBioscience
인간 및 원숭이 CD152(CTLA-4)를 인식하는 Biotin 결합 단클론 항체. 활성화된 T세포의 CTLA-4 발현 분석에 적합하며, Flow Cytometry 등에 사용 가능. 0.5 mg/mL 농도, 4℃ 보관, 동결 금지. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 0.06 µg/test | 13 publications |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rhesus monkey |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14D3 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466621 |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
- Unconjugated
- APC
- APC-eFluor 780
- Brilliant UV 737
- Brilliant UV 805
- eFluor 660
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The 14D3 monoclonal antibody reacts with human CD152 (CTLA-4), a protein structurally similar to CD28. CTLA-4 is expressed on activated T cells (and sometimes B cells) and binds CD80 (B7-1) and CD86 (B7-2) with higher affinity than CD28.
CTLA-4 and CD28 deliver opposing signals: CD28 acts as a costimulator, while CTLA-4 inhibits IL-2 secretion and T cell proliferation, restricting activation. The cytoplasmic region of CTLA-4 contains ER retention motifs, leading to intracellular localization of newly synthesized CTLA-4 in response to TCR signaling.
This antibody also recognizes rhesus monkey CTLA-4 and exhibits inhibitory activity.
Applications Reported: Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and tested using intracellular staining and flow cytometry of stimulated human peripheral blood cells with the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
Recommended usage ≤ 0.06 µg/test (100 µL final volume). Adjust cell number empirically (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.
Due to intracellular localization, full detection of CTLA-4 may require both surface and intracellular assessment.
Target Information
CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor primarily expressed on CD4+ T cells. It binds CD80 and CD86 with higher affinity than CD28, delivering inhibitory signals that suppress T cell activation. Intracellular CTLA4 is also found in regulatory T cells and contributes to their immunosuppressive function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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