Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), Biotin, eBioscience
마우스 CD152(CTLA-4)를 인식하는 단클론 항체로, Flow Cytometry에 적합합니다. Biotin으로 표지되어 있으며, 세포 내 및 세포 표면 CTLA-4 검출에 모두 활용 가능합니다. 0.5 mg/mL 농도의 액상 형태로, 4°C 암소 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 1 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UC10-4B9 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466619 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Formats
Product Specific Information
The UC10-4B9 monoclonal antibody reacts with mouse CD152, also known as cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4 is structurally similar to CD28 and is expressed on activated T cells at low levels. It binds B7 family members CD80 (B7-1) and CD86 (B7-2) with higher affinity than CD28.
CTLA-4 and CD28 deliver opposing signals to T cells: CD28 acts as a costimulator, while CTLA-4 inhibits IL-2 secretion and cellular proliferation, restricting T cell activation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, leading to intracellular localization of newly synthesized CTLA-4 following TCR signaling.
Due to the intracellular localization of CTLA-4, complete detection may require assessment of both intracellular and surface expression.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on resting and activated mouse splenocytes. Recommended use ≤1 µg/test (100 µL final volume). Cell numbers may range from 10⁵–10⁸ cells/test. Optimize titration for best performance.
Target Information
CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor mainly expressed on CD4⁺ T cells. It binds CD80 and CD86 with higher affinity than CD28, but unlike CD28, CTLA4 inhibits T cell activation and function. Intracellular CTLA4 is also present in regulatory T cells and contributes to their function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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