Thermo Fisher Scientific CD154 (CD40 Ligand) Monoclonal Antibody (MR1), Biotin, eBioscience

Thermo Fisher Scientific CD154 (CD40 Ligand) Monoclonal Antibody (MR1), Biotin, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• View 3 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• eFluor 450
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The MR1 monoclonal antibody reacts with mouse CD154 (CD40 Ligand, CD40L), a 39 kDa transmembrane glycoprotein transiently expressed by activated T cells. CD154 binds CD40 on antigen-presenting cells (APCs) such as B cells, monocytes/macrophages, and dendritic cells, facilitating T cell–APC interactions and B cell activation.

For flow cytometric staining, stimulation of enriched T cells or CD4+ cells prior to staining with MR1 is recommended.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on stimulated mouse splenocytes
  • Recommended ≤ 0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
  • Titrate for optimal performance

Target Information

CD40 Ligand (CD40-L, CD154) is a membrane glycoprotein expressed on T cells. It stimulates B cell proliferation and immunoglobulin secretion, induces cytokine production and tumoricidal activity in monocytes, and co-stimulates activated T cells to produce IFN-γ, TNF-α, and IL-2.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.