Thermo Fisher Scientific His Tag (C-term) Recombinant Rabbit Monoclonal Antibody (25B6E11), MonoRab

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.

The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRab™ technology to generate high-affinity and specific monoclonal rabbit antibodies.
GenScript MonoRab™ Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit is specific to six histidine tags located at the C-terminal or internal regions of fusion proteins.
This antibody enhances the effectiveness of various immunoassays, aiding researchers in identifying, detecting, and purifying polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

Target Information

The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at either the N- or C-terminal regions of recombinant proteins to facilitate isolation or purification by immobilized metal affinity chromatography (IMAC).

Epitope tagging involves fusing a known epitope to a recombinant protein via genetic engineering, enabling detection of proteins lacking specific antibodies.
His-tagged proteins, containing histidine stretches at the carboxyl or amino terminus, are purified through metal chelate chromatography due to the metal-binding properties of histidine clusters.
The strong and selective affinity between histidine-tag motifs and Ni²⁺ ions allows purification of proteins to homogeneity using Ni²⁺-NTA adsorbents.
His tag-specific antibodies are valuable tools for detecting or co-immunoprecipitating tagged proteins.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.