Thermo Fisher Scientific VISTA Monoclonal Antibody (VA.F6)

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

2-10 µg/mL

-

ELISA (ELISA)

0.01-0.1 µg/mL

-

Product Specifications

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

VA.F6

Immunogen

Recombinant human VISTA-Fc

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

VISTA is a negative immune checkpoint regulator that suppresses T-cell activation, and highly expressed on hematopoietic cells and within the tumor microenvironment. By analogy to VISTA and PD-L1 blockade, VISTA blockade may offer an immunotherapeutic strategy for human cancer. Therapeutic antibody against VISTA activates T cell signal and offers a way to promote this blockade. GenScript Human VISTA Antibody (VA.F6), mAb, Mouse is produced from a hybridoma resulting from the fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with recombinant human VISTA.

Target Information

VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1 it has a different expression pattern. In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells with the highest densities on myeloid cells, and lower levels on T cells. It is usually absent on B cells. VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy. The reported ligands of VISTA include CD28H, VSIG-3 and VSIG-8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.